IGEM:Indian Institue of Technology Madras/2009/Notebook/PLASMID - Plasmid Locking Assembly for Sustaining Multiple Insert DNA/2009/08/25

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  • Miniprep of the following:
  • J + S1; J+S2; I13602 and I13600.
  • Digestion(E digestion) of these parts and Gel run was performed
  • Gel order:
  • J+S1(1,1);J+S1(1,2);J+S1(1,3);J+S1(2,1);J+S1(2,2);J+S1(2,3);J+S2(1,1);J+S2(1,3);Ladder;J+S2(2,1);J+S2(2,3);I13602(1,1);I13602(1,2); I13600(2,1);I13600(2,2)
  • No DNA seen for any part at all.
  • Ordered primers for the final constructs.