IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey Construct/Implementation

From OpenWetWare
Jump to: navigation, search
Super Parts Molecular Prey-Predator Oscillator
Actual Part J37015page.JPG
Sub Parts Test Sensing Prey Construct <bbpart>C0261</bbpart> <bbpart>I13401</bbpart>


Ligation Strategy

Ligating the Prey Construct:

For Stage 1 Stage 2
<bbpart>J37034</bbpart>=
(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>)
C0261 logo.pngPlus sign.pngI13504 logo.png
<bbpart>J37015</bbpart>=
(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>)
J37015 logo.png


Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37034</bbpart>
J37034 logo.png Yes Yes Primers used
<bbpart>J37015</bbpart>
J37015 part.png Yes Yes Primers used

WetLab work entries

  • Part has been successfully built.
  • Pops blocker (for control of positive feedback loop) has been successfully made and needs to be incorporated into the prey part and tested.

<html> <!-- Start of StatCounter Code --> <script type="text/javascript" language="javascript"> var sc_project=1999441; var sc_invisible=1; var sc_partition=18; var sc_security="18996820"; </script>

<script type="text/javascript" language="javascript" src="http://www.statcounter.com/counter/frames.js"></script><noscript><a href="http://www.statcounter.com/" target="_blank"><img src="http://c19.statcounter.com/counter.php?sc_project=1999441&amp;java=0&amp;security=18996820&amp;invisible=1" alt="website statistics" border="0"></a> </noscript> <!-- End of StatCounter Code --> </html>