IGEM:IMPERIAL/2006/project/Oscillator/project browser/Test Sensing Prey Construct

Super Parts	Prey Construct
Actual Part	Error creating thumbnail: File missing T9002: Test Sensing Prey Construct
Sub Parts	F2620	intermediate_part	intermediate_part

• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation

Motivations

• This testing part, the Test Sensing Prey Construct, is representing the sensitivity of the prey cell towards the prey molecule (AHL)
• This part allows us to quantise the activation of gene expression by different concentrations of AHL and is used to assay AHL
• This testing part allows us to investigate three parameters:
  • How different AHL levels activate genes at a constant LuxR expression (The transfer function of pLuxR)
  • How much AHL is present, by referring to the transfer function
  • How LuxR levels effect gene activation

Part interface

INPUTS OUTPUTS Prey Molecule PoPs

Error creating thumbnail: File missing

Expected Behavior and Performance

System level specifications

Properties	Specifications
Input Range(s)	Input range of the prey should cover the total dynamic range of the prey.
Output Range	Output range of the PoPs can be any range, needs to be measurable with Wallac Victor III Fluorimeter.
Transfer function(s)	Error creating thumbnail: File missing
Latency	to be characterized
Robustness	...
Variability	Variability at the population level (day to day) should be as low as possible (10%)

Open issues

• list known issues