IGEM:Brown/2007/Lab Protocols

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/Biobrick assembly

/Preparing Antibiotic Stock Solutions

/Pouring Plates 1

/Pouring Plates 2

/Pouring Plates 3

/Autoclave Glassware

/10min E coli Miniprep

/Quick Tform into E. Coli from Qiagen prep DNA -from Brodsky

/Ultra Competent E coli prep - from Brodsky

/Ultra Competent E coli prep (updated 8/4/2007)- from Brodsky

/Ligation Setup-from Brodsky

/Preparing Ligation Backbone and Insert

/Constructing a Plasmid

/10X Ligation Buffer Recipe

/Creating Competent Cells -Jamie Gagnon

[| M9 Minimal Media (6/21/07)]