IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/07/13

iGEM Project name 1

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Glycerol Stock Confirmation PCR Protocol #11 Samples: pBad reverse, RBS RFP Terminator, J23100, RBS, pBad weak RBS RFP, RBS YFP Terminator Gel Electrophoresis 1% gel with SYBR green only pBad reverse, RBS RFP Term, and pBad weak RBS RFP, RBS were confirmed, to confirm J23100 on July 15 Material 1 reaction Master mix 10x buffer 2.5uL 24.75uL Vf2 primer 1.25uL 12.38uL Vr primer 1.25uL 12.38uL dNTP 0.5uL 4.95uL sd water 19.3uL 191.1uL Taq Pol 0.2uL 1.98uL DNA 0uL 0uL Total 25uL 247.5uL