IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/07/15

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Glycerol Stock Confirmation

  • PCR Protocol #11
  • Samples: J23100, RBS YFP Terminator, pBad strong RBS RFP, Amp Construction Plasmid, chlor construction plasmid, control
Gel Electrophoresis 1% gel with SYBR green all confirmed!
Material 1 reaction Master mix
10x buffer 2.5uL 16.5uL
Vf2 primer 1.25uL 8.25uL
Vr primer 1.25uL 8.25uL
dNTP 0.5uL 3.3uL
sd water 19.3uL 127.38uL
Taq Pol 0.2uL 1.32uL
DNA 0uL 0uL
Total 25uL 165uL