IGEM:British Columbia/2009/Notebook/Biosensor Sensitivity/2009/07/08

iGEM Project name 1

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Glycerol Stock Confirmation PCR Protocol #11 Samples: pBad reverse, RBS RFP Terminator, pBad strong RBS RFP, Amp Construction Plasmid, pBad weak RBS RFP, RBS YFP Terminator Gel Electrophoresis 1% gel with SYBR green only pBad reverse, RBS RFP Term, and pBad weak RBS RFP were confirmed, did not load enough sample, redo on July 13 Material 1 reaction Master mix 10x buffer 2.5uL 24.75uL Vf2 primer 1.25uL 12.38uL Vr primer 1.25uL 12.38uL dNTP 0.5uL 4.95uL sd water 19.3uL 191.1uL Taq Pol 0.2uL 1.98uL DNA 0uL 0uL Total 25uL 247.5uL