Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/05/08

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05/08/2015

Digested synthases: Aub, Bja, Bra, Cer, Esa, Las, Lux, Rpa, and Sin with E & X.
30uL protocol:

Heat inactivated at 80°C for 5 minutes and 65°C for 20 minutes.
These are in the freezer, ready to ligate with the backbone.