Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/05/11
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Today I ran a ligation with the PCR-purified, then double digested PCR synthase inserts + dephosphorylated and double digested backbone. Concentration of inserts was 200ng/30uL = 6.67ng/uL. I used this concentration to ligate at a 3:1 ratio. (47ng:50ng). Ryan finished up with a fast transformation.