Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/05/01

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05/1/2015

PCR Optimization of RhlI. 4 PCR tubes with DMSO, 4 PCR tubes without DMSO. Tubes were placed in the PCR machine with a temperature gradient. (range?)

PCR-purified synthase PCR products. Next, they are ready to be digested and then ligated with the backbone.

Sample Read# 260 280 260/280 ng/µL
Aub 0.025 0.014 1.814 24.637
Bja 0.033 0.017 1.938 33.411
Bra 0.045 0.025 1.782 44.594
Cer 0.033 0.018 1.852 33.122
Rpa 0.049 0.028 1.736 48.839
Esa 0.037 0.02 1.893 37.255
Lux 0.045 0.023 1.986 45.285
Las 0.033 0.019 1.802 33.354
Sin 0.024 0.013 1.8 23.961