Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/02/20

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02/20/2015

Based on the results of the gel we ran, there is reason to believe that the ligation worked in some of the colonies. So, I picked colonies 6, 8, 9, and 10. 6 and 8 are from the 1:3 plate and 9 and 10 are from the 3:1 plate. I put them in liquid cultures and placed them in the shaker overnight to grow.

Wu gel ligation.png