Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/02/23

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02/23/2015

Today I prepared the liquid colonies that I made on Friday for sequencing. I transferred the liquid to 2mL tubes and spun the tubes, poured out the LB broth and isolated the pellet of cells. Then I mini prepped them. The following shows the concentrations of each tube after the mini-prep.

Sample Read# 260 280 260/280 ng/µL
6 0.06 0.033 1.832 60.226
8 0.06 0.033 1.789 59.697
9 0.058 0.032 1.792 57.778
10 0.059 0.033 1.785 59.318


Rene sent the samples out for sequencing later in the day.