Haynes Lab:Notebook/CRISPR Editing/2016/08/30

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08/30/2016

Sonicated all the chromatin!

Luc14 g031 fixed 8/27/16
Luc14 g034 fixed 8/27/16
Gal4+puro g031 fixed 8/27/16
Gal4+puro g034 fixed 8/27/16
Gal4+dox g031 fixed 8/16/16
Gal4+dox g034 fixed 8/16/16



cell lysis, nuclei lysis
three rounds of 5 cycles of 15s on 45s off
Added ice each time

took 50ul from each, started uncrosslinking to confirm sonication on gel