Haynes Lab:Notebook/CRISPR Editing/2016/08/31

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08/31/2016

Finished prepping sonicated chromatin from yesterday to test on a gel. Gel looked alirght, started ChIPs for

Gal4 +puro untreated
Luc14 g031
Luc14 g034

50ug of chromatin per pulldown, checked concentrations today
Will do all the replicates on the same day to try to reduce error


31

53
55
50


new: 38
34

45
51
40


new:64

combined the old ones, will have plenty to do Gd34, which is the chromatin prep that didn't work yesterday. Perfect!
Gd 31 old won't have enoguh but I can combine it with the new chromatin prep and have plenty.

To double check that the chromatin is alright, I took 10ul of each to test on a gel. Started uncrosslinking at 5:20pm