Finished prepping sonicated chromatin from yesterday to test on a gel. Gel looked alirght, started ChIPs for
- Gal4 +puro untreated
- Luc14 g031
- Luc14 g034
50ug of chromatin per pulldown, checked concentrations today
Will do all the replicates on the same day to try to reduce error
combined the old ones, will have plenty to do Gd34, which is the chromatin prep that didn't work yesterday. Perfect!
Gd 31 old won't have enoguh but I can combine it with the new chromatin prep and have plenty.
To double check that the chromatin is alright, I took 10ul of each to test on a gel. Started uncrosslinking at 5:20pm