Haynes Lab:Notebook/CRISPR Editing/2016/08/29

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08/29/2016

Sonicated Gal4+puro cells fixed from a T-175 flask, had about 100ul of cells, brought up to the higher volumes for cell lysis. Sonicated for 3 rounds of 5 cycles of 15s on, 45 off, adding ice between each round. Took 100ul to check efficiency, put at 65degC. Snap froze the rest and put at -80.



Checked concentration of sonicated chromatin, ~380ng/ul. Should have plenty to do the pulldowns in triplicate.