Haynes Lab:Notebook/CRISPR Editing/2015/10/10

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Purified PCR product g031 EcoR1 donor

260 280 260/280 ng/µL
0.064 0.035 1.825 63.731

Luciferase assay of gal4 with puro or dox added at two times, 10/6 and 10/1. Dox added earlier didn't show an increase in silencing.

Transfected gal4-eed open and closed chromatin with

750ng of g031 + 250ng of g031 EcoRI donor
750ng of g034 + 250ng of g034 EcoRI donor

antibiotic free media