Haynes Lab:Notebook/CRISPR Editing/2015/10/10

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10/10/2015 Purified PCR product g031 EcoR1 donor 260 280 260/280 ng/µL 0.064 0.035 1.825 63.731 Luciferase assay of gal4 with puro or dox added at two times, 10/6 and 10/1. Dox added earlier didn't show an increase in silencing. Transfected gal4-eed open and closed chromatin with 750ng of g031 + 250ng of g031 EcoRI donor 750ng of g034 + 250ng of g034 EcoRI donor antibiotic free media