Haynes Lab:Notebook/CRISPR Editing/2015/10/09

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10/09/2015

Surveyor of siRNA experiment with g034, 1ul of surveyor enzyme, 1ul of enhancer, gently mixed, 42 deg for 60 min, 1.9ul stop solution, ran on BA

PCR with phusion, g031 EcoRI donor with P296/297, 4 50ul rxns, ann temp 71

Annealed g054 to test with new surveyor enzyme

Cell type drug gRNA rep ng/µL ul for 400 ul ann buff ul water
Gal4-EED puro g054 1 100.189 3.99 1.5 9.51



Plated cells for HDR experiment tomorrow
Picked more g034 colonies for miniprepping tomorrow