Haynes Lab:Notebook/CRISPR Editing/2015/10/11

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10/11/2015

Surveyor of g055 and test of g054, 42deg for 1h, 1.9ul stop solution, dilute 1:20, run on BA

Set up phusion PCR of sheared Luc14 gDNA with

P160/238
P161/233
P237/239


annealing temp 60, ext time 2min

changed media on transfected cells to media with antibiotics (including dox or puro)