Haynes Lab:Notebook/CRISPR Editing/2015/10/07

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10/07/2015 PCR purification Cell type drug siRNA drug gRNA rep 260 280 260/280 ng/µL ul for 400ng ul ann buff ul water Gal4-EED dox EZH2 puro g025 1 0.104 0.057 1.834 104.399 3.83 1.50 9.67 Gal4-EED dox EZH2 puro g025 2 0.087 0.046 1.876 86.528 4.62 1.50 8.88 Gal4-EED dox EZH2 puro g025 3 0.096 0.052 1.821 95.567 4.19 1.50 9.31 Gal4-EED dox none puro g025 1 0.117 0.063 1.863 116.936 3.42 1.50 10.08 Gal4-EED dox none puro g025 2 0.077 0.043 1.813 77.314 5.17 1.50 8.33 Gal4-EED dox none puro g025 3 0.088 0.046 1.901 88.068 4.54 1.50 8.96 Cell type drug siRNA drug gRNA rep 260 280 260/280 ng/µL ul for 400ng ul ann buff ul water Gal4-EED dox EZH2 puro g034 1 0.054 0.031 1.755 54.126 7.39 1.50 6.11 Gal4-EED dox EZH2 puro g034 2 0.063 0.035 1.829 63.131 6.34 1.50 7.16 Gal4-EED dox EZH2 puro g034 3 0.054 0.032 1.706 54.267 7.37 1.50 6.13 Gal4-EED dox none puro g034 1 0.068 0.038 1.81 68.014 5.88 1.50 7.62 Gal4-EED dox none puro g034 2 0.052 0.029 1.811 52.032 7.69 1.50 5.81 Gal4-EED dox none puro g034 3 0.05 0.028 1.777 50.273 7.96 1.50 5.54 g034 EcoRI donor 260 280 260/280 ng/µL 0.132 0.071 1.864 132.19