Haynes Lab:Notebook/CRISPR Editing/2015/10/06

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10/06/2015

Ran PCR of Gal4 siRNA g025 g034 samples with P197/198 on gel

no bands in neg ctrl, all other lanes look great



Col pur Gal4 puro/dox g054 samples, set up annealing reaction

Cell type drug gRNA rep 260 280 260/280 ng/µL ul for 400 ul ann buff ul water
Gal4-EED puro g054 1 0.1 0.054 1.87 100.189 3.99 1.50 9.51
Gal4-EED puro g054 2 0.09 0.047 1.91 90.466 4.42 1.50 9.08
Gal4-EED puro g054 3 0.105 0.057 1.862 105.5 3.79 1.50 9.71
Gal4-EED dox g054 1 0.104 0.054 1.919 103.727 3.86 1.50 9.64
Gal4-EED dox g054 2 0.115 0.063 1.825 114.985 3.48 1.50 10.02
Gal4-EED dox g054 3 0.092 0.049 1.891 92.038 4.35 1.50 9.15



Col pur Gal4 puro/dox g055 samples, set up annealing reaction

Cell type drug gRNA rep 260 280 260/280 ng/µL ul for 400 ul ann buff ul water
Gal4-EED puro g055 1 0.068 0.035 1.93 68.229 5.86 1.50 7.64
Gal4-EED puro g055 2 0.072 0.038 1.87 71.618 5.59 1.50 7.91
Gal4-EED puro g055 3 0.079 0.042 1.882 78.826 5.07 1.50 8.43
Gal4-EED dox g055 1 0.07 0.038 1.839 69.741 5.74 1.50 7.76
Gal4-EED dox g055 2 0.074 0.039 1.875 73.714 5.43 1.50 8.07
Gal4-EED dox g055 3 0.074 0.042 1.773 74.442 5.37 1.50 8.13