Haynes Lab:Notebook/CRISPR Editing/2015/10/03

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10/03/2015

PCR of siRNA samples wiht P197/198

Cell type drug siRNA gRNA rep ng/µL ul for 200ng 5ul water-200ng
Gal4-EED dox/puro EZH2 g025 1 179.613 1.11 3.89
Gal4-EED dox/puro EZH2 g025 2 173.433 1.15 3.85
Gal4-EED dox/puro EZH2 g025 3 104.127 1.92 3.08
Gal4-EED dox/puro Blank g025 1 201.109 0.99 4.01
Gal4-EED dox/puro Blank g025 2 233.53 0.86 4.14
Gal4-EED dox/puro Blank g025 3 174.093 1.15 3.85
Gal4-EED dox/puro EZH2 g034 1 96.926 2.06 2.94
Gal4-EED dox/puro EZH2 g034 2 62.154 3.22 1.78
Gal4-EED dox/puro EZH2 g034 3 123.599 1.62 3.38
Gal4-EED dox/puro Blank g034 1 214.213 0.93 4.07
Gal4-EED dox/puro Blank g034 2 213.397 0.94 4.06
Gal4-EED dox/puro Blank g034 3 182.355 1.10 3.90