Erickson:Protocols

PCR

Colony PCR
Real-Time PCR
PCR Clean-up for Sequencing
2-log Ladder

RNA

RNA Isolation Protocol (TRIzol)

RNA Isolation Protocol (Mini Shredder)

Fleas

Flea Bedding
Flea Crushing

Media

LB mixture (includes plates mix)
LB Buffer
TAE Buffer
TB
Frozen Stocks

Antibiotics

Kanamycin
Carbenacillin
Tetracycline
Irgasan
Chloramphenicol

Transposon Mutagenesis

Tri-parental Mating

Electroporation

Bi-parental Mating

Mutant Generation

File:Making a mutant.pptx

Competent Cell Generation

Competent Cells