Erickson:Colony PCR

From OpenWetWare

Colony PCR

  • PCR Master Mix
    1 μL Platinum Taq®
    30 μL 10X PCR Buffer (-MgCl2)
    5 μL Primers (forward and reverse)  (10 μL total)
    6 μL 50 mM MgCl2
    5 uL PCR nucleotides
    198 μL H2O
    250 μL volume total.  Adjust volume according to needs.

- For colony PCR, scrape small volume of colony and add to PCR Master Mix (20 μL)