Drummond:Protocols

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Local Protocols

Here are some protocols we use in the lab.

  • Chloroform/methanol precipitation of proteins
  • Resolving and detecting ubiquitin
  • Using paromomycin to alter translation in yeast
  • Cell preparation and protocol to view RFP/TFP fluorescence
  • Using the Zeiss LSM 510 confocal microscope
  • Yeast OD and cell viability model
  • Standard conditions for flow cytometer growth rate assay
  • High-efficiency yeast transformation
  • Yeast genomic DNA prep (consider the rather rapid yeast genomic DNA prep instead)
  • Isolating total, soluble and insoluble protein fractions
  • Making chemically competent E. coli cells
  • Preparing anti-ubiquitin beads
  • Periplasmic protein prep from E. coli

Remote Protocols

  • Spore enrichment for random-spore analysis (from Rockmill Meth. Enz. 1991)
  • Rather rapid yeast genomic DNA prep (Gottschling Lab)
  • SDS-PAGE (Szostak Lab)

Data Analysis Protocols

  • Akashi's test for translational accuracy selection

Notes

  • The relationship between the selection coefficient and relative growth rates: under construction
  • Thoughts on lysis of yeast cells
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  • This page was last edited on 13 October 2012, at 07:47.
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