Citizen Science/Open Fluorometer Project/Resources

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Resources

Vincent 10:05, 6 June 2009 (EDT): to be updated over the summer 2009 Feel free to add more relevant resources.

Glossary

• Fluorescence-related:
  • Fluorescence
  • Fluorophore
  • Emission spectra
  • Excitation spectra
• Optics-related:
  • LED
  • Lense
  • Light source collimation
  • Excitation filter
  • Emission filter
  • Dichroic mirror
  • Photodiode
• Electronic-reated
  • Lock-in system
  • Amplifier

Fluorescence measurements and standards

• Great tutorial for beginners
• Tutorial on Fluorescence and Fluorescent Instrumentation (PDF from Univ Washington)
• Standardization of Fluorescence Measurements: Criteria for the Choice of Suitable Standards and Approaches to Fit-for-Purpose Calibration Tools
• Standardization and Quality Assurance in Fluorescence Measurements: Techniques (Book)

Generic Fluorometer Designs

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Reviews

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Interesting published designs

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• "An Inexpensive LED-Based Fluorometer Used to Study a Hairpin-Based DNA Nanomachine", Hanwen Yan, 2005.

Selected designs (in details)

• Novak's design (2007)
• Shrinivasan's design (2006)
• Yang's design (2009)

Optics

• Equipment: lenses, dichroic mirrors, optical filters
• Thorlabs (great quality, but expensive)
• Comar (Cambridge, UK)
• check on ebay

Electronics

• Equipment: LEDs, microcontroller
• Lock-in system based on a 555 Timer IC for example

Commercial products

• Qubit from Invitrogen
• TBS-380 Mini-Fluorometer from Turner BioSystems
• SpectroFluorometer from Stellar Net Inc.
• Nanodrop Fluorometer