Citizen Science/Open Fluorometer Project/Resources

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Open Fluorometer Project

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Resources

Vincent 10:05, 6 June 2009 (EDT): to be updated over the summer 2009 Feel free to add more relevant resources.

Glossary

  • Fluorescence-related:
    • Fluorescence
    • Fluorophore
    • Emission spectra
    • Excitation spectra
  • Optics-related:
    • LED
    • Lense
    • Light source collimation
    • Excitation filter
    • Emission filter
    • Dichroic mirror
    • Photodiode
  • Electronic-reated
    • Lock-in system
    • Amplifier


Fluorescence measurements and standards

Generic Fluorometer Designs

Open Fluorometer Design 1.png Open Fluorometer Design 2.png

Reviews

  1. Xiao D, Zhao S, Yuan H, and Yang X. CE detector based on light-emitting diodes. Electrophoresis. 2007 Jan;28(1-2):233-42. DOI:10.1002/elps.200600473 | PubMed ID:17154328 | HubMed [1]
  2. Xiao D, Yan L, Yuan H, Zhao S, Yang X, and Choi MM. CE with LED-based detection: an update. Electrophoresis. 2009 Jan;30(1):189-202. DOI:10.1002/elps.200800415 | PubMed ID:19072930 | HubMed [2]
All Medline abstracts: PubMed | HubMed

Interesting published designs

  1. Yang FB, Pan JZ, Zhang T, and Fang Q. A low-cost light-emitting diode induced fluorescence detector for capillary electrophoresis based on an orthogonal optical arrangement. Talanta. 2009 May 15;78(3):1155-8. DOI:10.1016/j.talanta.2009.01.033 | PubMed ID:19269486 | HubMed [1]
  2. Novak L, Neuzil P, Pipper J, Zhang Y, and Lee S. An integrated fluorescence detection system for lab-on-a-chip applications. Lab Chip. 2007 Jan;7(1):27-9. DOI:10.1039/b611745g | PubMed ID:17180202 | HubMed [2]
  3. Shrinivasan S, Norris PM, Landers JP, and Ferrance JP. A low-cost, low-power consumption, miniature laser-induced fluorescence system for DNA detection on a microfluidic device. Clin Lab Med. 2007 Mar;27(1):173-81. DOI:10.1016/j.cll.2006.12.010 | PubMed ID:17416310 | HubMed [3]
  4. Segura-Carretero A, Fernández-Sánchez JF, and Fernández-Gutiérrez A. Simple luminescence detector for capillary electrophoresis. Methods Mol Biol. 2009;503:221-37. DOI:10.1007/978-1-60327-567-5_12 | PubMed ID:19151944 | HubMed [4]
All Medline abstracts: PubMed | HubMed
  • "An Inexpensive LED-Based Fluorometer Used to Study a Hairpin-Based DNA Nanomachine", Hanwen Yan, 2005.


Selected designs (in details)

Optics

Electronics

  • Equipment: LEDs, microcontroller
  • Lock-in system based on a 555 Timer IC for example

Commercial products