Chang Lab:Notebook/CBE/08/149/2008/12/04

From OpenWetWare
Jump to: navigation, search
<html> <a href="http://ntu.edu.sg/"> <IMG SRC="http://openwetware.org/images/4/41/NTULogo.jpg" align="left"></a> </html>
Antimicrobial efficacy testing of antibiotic-containing biodegradable nanopolymers against biofilm and planktonic cells
<html><img src="/images/9/94/Report.png" border="0" /></html> Main Notebook page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Logs

  • Time in: 0930 hrs
  • E coli culturing
    • Biosafety Hood UV-irradiated for 20 mins, turned off and left for 5 mins (SOP)
    • 3/12 E coli culture removed from shaking incubator (incubate 16 hrs)
    • Under the hood, one loopful of E coli streaked (quadrant streaking method) onto LB(A) plate (as immediate culture)
    • Under the hood, one loopful of E coli streaked (quadrant streaking method) onto LB(A) slant (as stock culture)
    • 2X E coli plate and slant incubated at 37 C overnight (time start: 1020 hrs)
  • Medium preparation
    • Repeat of 3/12 procedure [1]

Notes

  • Always Parafilm petri dishes when storing
  • Overturn dishes upon storing (for incubation, overturn only when no moisture is visibly found)