Chang Lab:Notebook/CBE/08/149/2008/12/05

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Antimicrobial efficacy testing of antibiotic-containing biodegradable nanopolymers against biofilm and planktonic cells
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Logs

  • Started at 0945am.
  • Culture medium
    • 1g of BD Agar and 11g of BD Broth weighed out on a weighing scale into a 1000ml flask.
    • Add 200ml of distilled water
    • Add 400ml of BD Agar solution (14g BD Agar to 400ml distilled water) and 400ml of BD Broth (14g LB Broth to 400ml distilled water).
    • Stir well and separate into 3 bottles (300ml each).
  • Autoclaved 1030am at 121 degrees for 15min (waited for about 2hrs for the temperature to rise and pressure to build up and lower). Temperature safe to remove is 80 degrees.
  • Prepared E coli (K12 W3110) broth mixture and incubated at 30 degrees.
    • E coli was filled with LB Broth in a test tube (ratio 0.1ml:9ml).
    • Placed in a shaking incubator at 252rpm for 6hr at 35.2 degrees (incubated from 10am - 5 pm).
  • Culture streak
    • Streaked 2 LB agar plates using E coli broth culture for overnight incubation at 37.0 C.