Biomod/2012/Columbia/KinesinKings/Overview

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<div id="nav"> <h1 id="sitename"><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings">Kinesin Kings</a></h1> <u1 id ="menu"> <ul class="blockeasing"> <li class="main"><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/AboutUs" onmouseover="this.style.borderBottomColor ='#aaaaaa'"

  onmouseout="this.style.borderBottomColor = '#123456'">About Us</a>

<ul class="subnav"> <li><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/AboutUs#Undergraduates">Undergrads</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/AboutUs#Graduate_Student_Mentors">Graduate Mentor</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/AboutUs#Faculty_Mentor">Faculty Mentor</a></li> <!-- <li><a href="#">Idea</a></li> --> </ul> </li> <li class="main"><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/Extras">Extras</a> <ul class="subnav"> <li><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/Extras#">3D Image</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/Extras#References">References</a></li> <!-- <li><a href="#">Grad</a></li> --> <!-- <li><a href="#">Columbia</a></li> --> </ul> </li> <li class="main"><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/Project">Project</a> <ul class="subnav"> <li><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/Project#Materials">Materials and Methods</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/Project#Results">Results</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/Project#Discussion">Discussion</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/Project#Significance">Future Applications</a></li> </ul> </li>

                             <li class="main"><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/Overview">Overview</a>

<ul class="subnav"> <li><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/Overview#Objectives">Objectives</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/Overview#Abstract">Abstract</a></li> <!-- <li><a href="#">LinkedIn</a></li> --> <!-- <li><a href="#">Facebook</a></li> --> </ul> </li>

                             <li class="main"><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/Background">Background</a>

<ul class="subnav"> <li><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/Background#Definitions">Definitions</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2012/Columbia/KinesinKings/Background#Current_Practices">Common Practices</a></li> <!-- <li><a href="#">Scientific Knowledge</a></li> -->

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Objectives

Explore the effects of the amino acid chain poly-L-lysine on microtubules

Design a micro-mesh of microtubules by utilizing the unique effects of poly-L-lysine


Abstract

The polymerization of tubulin dimers results in the formation of long, hollow, and rigid structures known as microtubules. Current techniques involving microtubules and cargo transport situate the microtubules on a horizontal plane, parallel to a glass surface. We sought to create novel structures of microtubules in synthetic biology. We designed a 3D mesh of microtubules by coating the glass surfaces in a flow cell with the polymer poly-L-lysine. This caused the microtubules to orient themselves orthogonally to the glass surface and to form web-like structures spanning the height of the flow cell. We observed attachment due to electrostatic interactions between the rhodamine-labeled microtubules and the poly-L-lysine coated surfaces almost immediately in the flow cell. The poly-L-lysine in solution is conjectured to have acted as a bridging mechanism between the microtubules that created the observed structures. This new mesh has potential to add to the biosensing field in physical filtration devices.


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/*#menu li a span {text-decoration: underline;!important;}**/ .post { padding:8px; margin-bottom:10px;} .columns {padding:8px;} .columnleft {float:left; width:49%;} .columnright {float:right; width:49%;} .col {float:right; width:41%; margin-right:12px; margin-bottom:15px;} .col p {margin-bottom:10px!important;} .txtbutton a{display:block; background:url(images/learnmore.jpg) no-repeat!important; height:19px; width:90px; text-align:center; font:bold 12px "Trebuchet MS", Arial, Helvetica, sans-serif; color:#ccc!important; text-shadow:#000 1px 1px 1px; text-decoration:none; padding:2px 0 0 0!important;} .txtbutton a:visited, .txtbutton a:active {display:block; background:url(images/learnmore.jpg) no-repeat!important; height:19px; width:90px; text-align:center; font:bold 12px "Trebuchet MS", Arial, Helvetica, sans-serif; color:#ccc!important; text-shadow:#000 1px 1px 1px; text-decoration:none; padding:2px 0 0 0!important;} .txtbutton a:hover {background:url(images/learnmore-hover.jpg) no-repeat!important; color:#fff!important;}

  1. bottombar {

background:#000;width:770px; position:fixed; bottom:0; left:50%; height:25px; margin:0 0 0 -385px; z-index:998; }

.categories li {display:block; height:28px; font: bold 16px "Trebuchet MS", Arial, Helvetica, sans-serif;} .categories li a {display:block; background:url(images/catgory1.jpg) no-repeat; width:254px; height:22px; padding:4px 10px 0 10px; text-shadow: #000 1px 1px 1px; color:#555;} .categories li a:visited, .categories li a:active {color:#555;} .categories li a:hover {background:url(images/catgory2.jpg) no-repeat; color:#fff;}

  1. sidebar ul {margin-bottom:10px;}

.block { width:274px; margin-bottom:10px;} .blocktop{background: #ffffff /*url(images/darkbox1.jpg) no-repeat; height:6px;*/} .blockmid {padding:8px; background: #ffffff/*url(images/darkbox2.jpg) repeat-y;*/} /*.blockfooter {background:url(images/darkbox3.jpg) no-repeat; height:6px;}*/ p.ads {display:block;} img, a img {border:none;} p.ads img {display:block; float:left; margin:2px;} .breadcrumb {display:block; width:630px; float:left; padding:12px 0 0 10px;} .breadcrumb li { display:inline; font:bold 12px "Trebuchet MS", Arial, Helvetica, sans-serif; text-shadow: #000 1px 1px 1px;}

  1. searchbar{margin-left:665px; padding:6px 0 0 0;}
  2. searchbar div { float:left; margin-right:5px;}
  3. searchbar input {color:#fff;}
  4. searchbar .keyword {display:block; background: transparent url(images/searchfield.jpg) no-repeat; width:185px; height:27px; padding:5px 5px 0 5px; border:none; float:left; color:#fff;}
  5. sidebar .posts {padding:5px;}
  6. sidebar .posts li {display:block; padding:5px 0 5px 0;}
  7. sidebar .posts li span {font:11px "Trebuchet MS", Arial, Helvetica, sans-serif; color:#444; display:block;}
  8. sidebar .posts li a {display:block; border-bottom: dashed 1px #9EC5DA;}
  9. footerlinks {background:url(http://openwetware.org/images/6/60/Bottombar.jpg) repeat-x; height:41px;}
  10. bottom {background:#000; padding:5px;}

.alignleft {float:left;} .alignright {float:right;} .post .articleimg {padding:8px; background:#111; border:solid 1px #000;} img.alignleft { margin:0 15px 0 0;} img.alignright {margin:0 0 0 15px;} .post a {background:#171612; text-shadow:#000 1px 1px 1px; } .post a:hover {background:#000; color:#fff;}

  1. footernav {display:block; padding:5px 25px 5px 5px;}
  2. footernav li { display:inline; font:bold 12px "Trebuchet MS", Arial, Helvetica, sans-serif; text-shadow: #000 1px 1px 1px; padding:0px 0px 0px 0px;}

.credit {font:11px "Trebuchet MS", Arial, Helvetica, sans-serif; color:#444; display:block; padding:5px 0 5px 0;} h1 a, h2 a, h3 a, h4 a {background:none!important;}

  1. comments {padding:10px; background: #171612;}
  2. comments h2 {background:#000; padding:10px; font-size:18px!important;}
  3. comments .entry {padding:10px; background:#11100D; margin-bottom:10px;}
  4. comments .entry p {display:block; margin-bottom:8px!important;}
  5. comments .entry .avt {float:left; width:55px; margin-right:10px;}

.avt img {background:#000; padding:5px;}

  1. comments .entry .con, #comments .entry .name, #comments .entry .date { margin-left:70px;}
  2. comments .entry .con blockquote {font:italic 14px Georgia, "Times New Roman", Times, serif; color:#666; padding-left:15px!important; border-left: solid 5px #333!important; margin:0!important;}

h3#respond {color: #FC6;} .comments-form {background:#000; padding:10px;} .formid, .formuri, .formemail {padding:3px; height:25px; background:#171612; border:solid 1px #1C1B17; width:250px; color:#999; font-size:16px; } .comments-form label {padding-left:15px;} .comments-form textarea {padding:3px; background:#171612; border:solid 1px #1C1B17; color:#999; font-size:16px; } .postheader {margin-bottom:18px;} .postheader .postdetails {font-size:11px; color:#555;} .post blockquote {display:block; margin-left:10px; padding:5px 5px 5px 40px; border-left: solid 5px #333; font:14px italic Georgia, "Times New Roman", Times, serif; background:url(images/quote.jpg) 5px 0 no-repeat; margin-bottom:18px;} cite {display:block; color:#aaa; font-weight:bold;} .post blockquote p {margin-bottom:0!important;} blockquote.alignleft {width:200px;} blockquote.alignright {width:200px;} .post ul, .post ol {margin-bottom:18px;} .post ul li {display:block; padding:3px; line-height:16px; background:url(images/bullet.gif) 5px 5px no-repeat; padding-left:20px; margin-bottom:10px;} .post ol { margin-left:25px;} .post ol li { padding:3px; line-height:16px; margin-bottom:10px;} .post code {display:block; white-space:pre; background:#171612; padding:5px; margin-bottom:18px; font:12px "Courier New", Courier, monospace; border: solid 1px #312F29;}

.tags {padding:8px; background:#171612; margin-bottom:18px;}

.authorinfo {padding:8px; border-top: dashed 1px #333; border-bottom: dashed 1px #333; margin-bottom:18px;} .authorinfo img {float:left; margin-right:15px;} .postinfo {font-size:11px; font-weight:bold; display: block; margin-bottom:18px; font-family:"Trebuchet MS", Arial, Helvetica, sans-serif;} .postbottom { border-bottom:dashed 1px #000; margin-bottom:18px; padding-bottom:8px;}

.wp-pagenavi {display:block; height:30px; margin-bottom:18px; text-shadow: #000 1px 1px 1px;} .wp-pagenavi span, .wp-pagenavi a {display:block; height:20px; padding:3px 8px 0 8px; text-align:center; float:left; background:#171612; margin-left:2px;}

.wp-pagenavi a:visited, .wp-pgenavi a:active {display:block; height:20px; padding:3px 8px 0 8px; text-align:center; float:left; background:#171612; margin-left:2px;} .wp-pagenavi .current, .wp-pagenavi a:hover {background:#000; color:#fff;}


</style>


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<a><img src ="http://openwetware.org/images/5/54/CULogo.png" width="181" height="35" alt="Columbia" style ="float:right; margin-right:-45px;"/></a></p>


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