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<div id="navigation"> <div id="menu" style="position:static"> <ul> <li><a class="aMain" href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo">Home</a></li> <li><a class="aTeam" href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Team/Students">Team</a></li> <li><a class="aProject" href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project">Project</a>  <li><font color="#ffffff">Results</font> <ul> <li><a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project/Results">Experiments</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project/Simulations">Simulations</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Achievements/DNA_Devices">DNA Design</a></li> </ul></li>  <li><a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project/Achievements">Achievements</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Project/Future_works">Future works</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Notebook/Protocols">Protocols</a></li> <li><a href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Notebook/Lab.notebook">Notes</a></li> <li><a class="aNotebook" href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Sponsors/">Sponsors</a></li> <li><a class="aSitemap" href="http://openwetware.org/wiki/Biomod/2011/TeamJapan/Tokyo/Sitemap">Sitemap</a></li>

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Tools

Software

<Sequence Design>

NUPACK: Software to design DNA arraignment.

→Direct Link:http://www.nupack.org/partition/browser

The DINAMelt Web Server: We used to know K_m of DNA strand.

→Direct Link:http://mfold.rna.albany.edu/?q=DINAMelt

<Software of editing>

Paint.net: Image processing software. We used it to control light and shade.

→Direct Link:http://www.paint.net/

Image J: Image analysis software. We used it to process photo of electrophoresis.

→Direct Link:http://rsbweb.nih.gov/ij/

Inkscape: We used it to draw figures.

→Direct Link:http://inkscape.org/index.php?lang=en

Chem Bio Draw: We used it mainly to draw chemical formula.

→Direct Link:http://www.cambridgesoft.com/software/details/?fid=15&pid=226

aviutl: We used it mainly to edit YouTube videos.

→Direct Link:http://www.gigafree.net/media/me/aviutl.html

trakAxPC: We used it mainly to edit YouTube videos.

→Direct Link:http://www.trakax.com/software/pc/download/

CyberLink power director: We used it mainly to edit YouTube videos.

→Direct Link:http://www.cyberlink.com/products/powerdirector/overview_en_US.html

Laboratory instrument

Micro fine machining center

Roland MODELA MDX-40A

http://www.rolanddg.com/product/3d/3d/mdx-40a/index.html

End mill

TECNOS Co, Ltd.

-RSE230-0.5X2.5---Diameter is 0.5mm

-RSE230-1X5---Diameter is 1mm

-RSE230-3X15---Diameter is 3mm

Mili-Q water

http://www.millipore.com/catalogue/item/zrqsvp0ww

Tabletop Centrifuge

[1](Only Japanese)

Ultracentrifuge

HITACHI himac CT 15RE

http://www.hitachi-koki.com/himac/centrifuges/ct15e/ct15e.html

Fluorescence Phase contrast microscope

OLYMPUS CKX41

http://www.microscopy.olympus.eu/microscopes/Life_Science_Microscopes_Routine_microscope_CKX41.html

Shaker

LMS SHM2001

http://www.lms.co.jp/commodity/cat1/cat0101/post_43.html

Rotator of micro tube

As One MTR-103

http://www.justis.as-1.co.jp/jus-tis/web/DetailEnglish.aspx?sid=justis&catalog=GJ&group=G025401&no=&op_from=jus-tis

Band Saw

Hozan K-100

http://www.hozan.co.jp/E/catalog/Metal_working/K100.html

DESICCATOR VACUUM

AS ONE 1-068-01

http://www.justis.as-1.co.jp/jus-tis/web/DetailEnglish.aspx?sid=justis&catalog=GJ&group=G007103&no=&op_from=jus-tis

Vacuum pumps

TGK LV-435A

http://www.tgk.co.jp/info/0904645105s.html

Camera

CASIO EX-F1

http://www.casio-intl.com/dc/ex_f1/

Heating Block

THERMO BLOCK ND-M01

http://www.tech-jam.com/scientific_research_equipment/incubator/kn3316700.phtml (Only Japanese)

Material Lists and Kits

Name	grade	Supplier	Product code	Cat No	Lot No
Sodium Hydroxide	Wako 1st Grade	Wako	198-13765	1310-73-2	LAN1989
SILPOT 184	***	DOW CORNING TORAY	03255981	***	0006410158
Acetone	Wako 1st Grade	Wako	012-00343	67-64-1	DCN6798
2-Propanol	Wako 1st Grade	Wako	166-04831	67-63-0	DCM6848
Ethnol(99.5)	Wako 1st Grade	Wako	057-00451	64-17-5	DBM6540
Albumin, from Bovine Serum, Cohn Fraction V, pH7.0	Biochmistry	Wako	013-23291	***	STF3372
Di(N-succinimidyl) Suberate	***	TCI	D3895	68528-80-3	CU3BM
Ultra PureTM 1M Tris-HCL pH 8.0	***	invitrogen^TM	15568-025	***	9949164
40(w/v)%-Acrylamide/Bis Mixed Solution (29:1)	SP	nacalai tesque	***	***	L1F7876
Dimethyl Sulfoxide	Wako 1st Grade	Wako	043-07216	67-68-5	LAL4398
Sodium Dodecyl Sulfate(SDS)	Wako 1st Grade	Wako	196-08675	151-4-3	LAN1411
SSC Buffer 20x Concentrate	***	SIGMA	***	S6639-1L	021M8403
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide Hydrochloride	SU	TCI	D1601	25952-53-8	FJXDI
N-Hydroxysuccinimide	***	TCI	H0623	6066-82-6	EOC3E
Suberic acid bis(3-sulfo-N-hydroxysuccinimide ester) sodium salt	***	SIGMA	S5799-25MG	***	051M4067V
SYBR Gold nucleic acid gel stain	***	life technologies^TM	S11494	***	927072
Dimethyl Sulfoxide	for Molecular Biology	Wako	041-29351	67-68-5	DCN0023
Zinc Sulfate	Practical Grade	Wako	268-00422	7733-02-0	LAQ6174
Urea	for Molecular Biology	Wako	211-01213	57-13-6	LAQ5967
Magnesium Chloride	***	Wako	136-03995	7786-30-3	LAR4676
Tris-Borate-EDTA Buffer (10X), Nuclease an Protease tested [TBE Buffer]	***	nacalai tesque	35440-31	***	L1A6455
Ammonium Peroxodisulfate	Wako 1st Grade	Wako	018-03282	7727-54-0	HLH7635
N,N,N',N'-TETRAMETHYL- ETHYLENEDIAMINE	Wako 1st Grade	Wako	202-04003	110-18-9	EPF1167
0.5N EDTA (pH8.0)	***	Wako	311-90075	***	01481B
Glycerol	Wako 1st Grade	Wako	075-00616	56-81-5	LAQ5416
Disodium Hydrogenphosphate 12-Water	Wako 1st Grade	Wako	196-02835	10039-32-4	LAQ5931
Sodium Dihydrogenphosphate Dihydrate	Wako 1st Grade	Wako	192-02815	13472-35-0	LAR4091
Hydrochloride Acid	Wako 1st Grade	Wako	080-01066	7647-01-0	LAN1065
PolyLink - Protein Coupling Kit for COOH Microparticles For Microparticles 1.0 Micron or Larger	***	Polysciences, Inc.	24350	***	631643
PolyLink EDAC	***	Polysciences, Inc.	2435C	***	631321

Biomod/2011/TeamJapan/Tokyo/Notebook/Tools

Tools

Software

<Sequence Design>

<Software of editing>

Laboratory instrument

Material Lists and Kits

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