BME100 s2017:Group7 W1030AM L5
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LAB 5 WRITE-UPPCR Reaction ReportThe prelab materials were beneficial in their specificity of demonstration for how exactly micropipetting works and what what the proper technique is. Additionally, having used the previous lab meeting time to prepare for the actual PCR aided in ensuring that the experiment was understood both conceptually and technically and thus able to be executed properly. Because the prelab materials allowed for a firm understanding of micropipetting technique, the difference between the first and second stop on the pipettor were understood and the amount of liquid in each reaction remained constant. Fluorimeter ProcedureImaging set-up The settings for the smartphone camera itself were as follows: flash turned off, ISO set to 800, white balance to auto, exposure and saturation to the highest setting, and contrast to the lowest setting. A black box was placed sideways on the table such that the opening at the top faced outward toward the experimenter in order; this box was used to block out any excess light. The LED light device was placed under the cover the box provided, with the stand for the camera of the smartphone placed 4 cm away from the LED device. The smartphone was then placed inside the stand.
Data Collection and AnalysisImages of High, Low, and Zero Calf Thymus DNA
PCR Results: Summary
For the 5μg/mL sample, the fluorimeter result had a bright white color. For the 0.5μg/mL, the fluorimeter result had a white hue to it, but was considerably darker than the 5μg/mL. In the 0μg/mL, the fluorimeter result had some white hues to it, but was considerably lass reflective and darker in color than the previous results.
The results of this patient were similar to those of the previous patient. For the 5μg/mL sample, the fluorimeter result had a bright white color. For the 0.5μg/mL, the fluorimeter result had a white hue to it, but was considerably darker than the 5μg/mL. In the 0μg/mL, the fluorimeter result had some white hues to it, but was considerably lass reflective and darker in color than the previous results. Conclusions
From the ladder on the left to the right the cells are: positive control, negative control, patient 1 replicate 1, patient 1 replicate 2, patient 1 replicate 3, patient 2 replicate 1, patient 2 replicate 2, patient 2 replicate 3. The patients had a negative result, as shown above in the gel electrophoresis. The only positive that was shown by the gel was the positive control, but in the end the patients had an overall negative result. |