BME100 s2014:W Group5 L2

BME 100 Spring 2014	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Juan B. Name: Naomi Walliman Name: Your name Name: Your name Name: Your name LAB 2 WRITE-UP Descriptive Statistics The purpose of this experiment is to find out the minimum LPS dosage required in order to have a significant effect on blood inflammotin concentration. We had a look at two broad groups in our study – humans and rats. These groups were then separated into subgroups according to treatment. Analysis of results is as follows: Data Inflammotin (pg/ml) Human 0mg 5mg 10mg 15mg 5.23 10.72 100.19 793.17 1.01 9.29 75.92 476.67 4.23 8.46 23.46 771.45 1.87 10.19 70.87 795.09 3.67 7.29 19.27 181.27 2.98 7.54 99.65 752.78 5.83 8.67 38.37 934.23 5.24 6.15 76.26 554.87 4.27 10.92 35.25 692.23 4.01 10.09 76.98 627.65 AVERAGE 3.834 8.932 61.622 657.941 Std. DEV 1.523010177 1.593931547 30.11069386 212.943 Std. Error 0.481618106 0.504045412 9.521837451 67.33848 Anova: Single Factor SUMMARY Groups Count Sum Average Variance 0mg 10 38.34 3.834 2.31956 5mg 10 89.32 8.932 2.540618 10mg 10 616.22 61.622 906.6539 15mg 10 6579.41 657.941 45344.71 ANOVA Source of Variation SS df MS F P-value F crit Between Groups 3027017 3 1009006 87.2536 1.4E-16 2.866266 Within Groups 416306 36 11564.06 Total 3443323 39 Grouping P-value Adjusted P-value Significant? 0-5 8.59631E-07 0.008333333 Y 0-10 9.94377E-06 0.008333333 Y 0-15 1.39436E-08 0.008333333 Y 5-10 3.01859E-05 0.008333333 Y 5-15 1.57101E-08 0.008333333 Y 10-15 6.4824E-08 0.008333333 Y Rats Inflammotin (pg/ml) Rats 0mg 5mg 9.24 22.34 8.76 6.45 8.78 14.23 13.5 3.55 12.3 8.99 Doses Average Standard Deviation Standard Error T-Test 0 mg 10.516 2.225551617 0.995296941 0.8674035 10 mg 11.112 7.402885924 3.310671231 Analysis Experiment 1 For our human trials, we had five different treatment groups receiving 0mg, 5mg, 10mg or 15mg of LPS. P-score analysis of every possible combination of these groups showed a statistically significant difference (p < 0.008333333) for each and every one of these pairings as well as the ANOVA. Based on these numbers we can predict that treatment will have an effect on blood inflammotin levels and that the higher the dosage (up to 15mg), the greater the response. Experiment 2 Rat trials were slightly less complicated as we only compared treatments of 0mg and 10mg of LPS. The p-value between these two groups is 0.867 – this is not statistically significant and so the treatment is deemed to have no effect.