BME100 s2014:W Group2 L6
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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LAB 6 WRITE-UP
The TinkerCAD tool is a comprehensive Computer-Aided Design system that allows for ease of use and complex 3 dimensional designs. The CAD is used for a variety of purposes, but the main purpose is 3D Printing. For the lab, We used TinkerCAD to construct our redesigned Open PCR apparatus.
Feature 1: Disease SNP-Specific Primers
Background on the cancer-associated mutation
Nucleotides are the basic building blocks of nucleic acids. A polymorphism is when different phenotypes exist in the same population
How the primers work: Based on the way the disease-specific primer is designed, the forward primer will bind 100% to a complementary
Feature 2: Consumables Kit
The open PCR machine, fluorimeter, and pipette/lab materials will all be packaged together in a small and convenient box. The PCR machine will come disassembled, but can easily be put together by following the step by step process found on the machine's website. The fluorimeter will have step by step instructions and an app for smartphones to guide one through the process.
Feature 3: Hardware - PCR Machine & Fluorimeter
The PCR machine will come disassembled in the package. An easy to follow, step-by-step instruction manual will be included to show how to put the machine together. The fluorimeter will come with instructions as well as a link to an app that leads one through the process. The app will have the pictures ready to use with Imagej on any computer system.
The PCR machine will have better interfacing options including a program built into the machine rather than connecting to a computer to
The fluorimeter will be redesigned with a phone cradle compatible for all phones to keep them in place. The phone cradle allows the
The current design of the PCR machine will be altered to address the weaknesses such as waste from plastic tips, keeping track of tubes,
The current design of the fluorimeter will be altered to address the weaknesses such as the unstable phone cradle, using items to prop up
Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach
The answer for calculation 4 is less than 1. The answer for calculation number 3 is also less than 1, but it is smaller than the result for calculation 4. That means that the probability that a patient will develop the disease, given a positive final test conclusion is smaller than the probability that a patient will not develop the disease, given a negative final test conclusion.