BME100 s2014:T Group6 L2

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OUR TEAM

Name: Doan-Nhi T. Tran
Name: Mark A. Keppler
Name: Rayan S. Altayyar
Name: Nicole D. Fisk
Name: Brittani M. Ogden

LAB 2 WRITE-UP

Descriptive Statistics

The Effect of Lipopolysaccharide in Human Subjects
Inflammotin Concentration by Dosage for 0mg Trial (pg/ml)
Mean: 3.834
Standard Deviation: 1.523
Standard Error: 0.482

Inflammotin Concentration by Dosage for 5mg Trial (pg/ml)
Mean: 8.932
Standard Deviation: 1.594
Standard Error: 0.504

Inflammotin Concentration by Dosage for 10mg Trial (pg/ml)
Mean: 61.622
Standard Deviation: 30.111
Standard Error: 0.9.522

Inflammotin Concentration by Dosage for 15mg Trial (pg/ml)
Mean: 657.941
Standard Deviation: 212.943
Standard Error: 67.338

The Effect of Lipopolysaccharide in Rat Subjects
Inflammotin Concentration by Dosage for 0mg (pg/ml)
Mean: 10.512
Standard Deviation: 2.226
Standard Error: 0.995

Inflammotin Concentration by Dosage for 10mg (pg/ml)
Mean: 11.112
Standard Deviation: 7.403
Standard Error: 3.311



Results

[Graphs unavailable]




Analysis

ANOVA Test on the Effect of Lipopolysaccharide in Human Subjects
In order to properly demonstrate the significance of our statistical correlations in human trials we

conducted the ANOVA test since we had 4 separate groups with 10 subjects per group. Each group

received a different dose of lipopolysaccharide incremented in 5 mg portions. ANOVA returned a p value

of 1.401E-16 signifying a confidence several orders of magnitude greater than the required 0.05 p value.

We followed up our analysis by including comparative T-Tests in order to demonstrate significance

between each possible combination of paired groups. All returned T-Test values were found to be

several orders of magnitude greater than the required confidence interval.


T-Test on the Effect of Lipopolysaccharide in Rat Subjects
In order to properly demonstrate the significance of correlation within our two rat trials we conducted

a T-Test which returned a p value of 0.867. This value does not correlate within our required interval

of 0.05 which demonstrates that the lipopolysaccharide did not significantly induce Inflammotin

accumulation.




Summary/Discussion

Lipopolysaccharide has been found to be significant in human trials with greater than 99.99% confidence

at all tested concentrations. Significance has also been demonstrated between test groups using T-Test

analysis for all possible pair combinations. Rat trials however only demonstrate a confidence of 13.26%

which does not meet the minimum 95% requirement.