BME100 s2014:T Group4 L6
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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LAB 6 WRITE-UP
Feature 1: Disease SNP-Specific Primers
Background on the disease-associated mutation
How the primers work:
Feature 2: Consumables Kit
The tray that held the tubes in the PCR machine could not hold very many tubes. To address this problem we enlarged the tube tray to be able to hold a much greater amount of tubes. This can greatly increase the rate of tubes that can be tested at once. The tubes themselves we kept the same size and shape. However the PCR machine had unused space on both sides of the tube holding tray. Therefore, on TinkerCAD we extended the tube holding tray inorder to add more slots for additional tubes.
The major weakness this redesign addresses it how long it takes the PCR machine to test only a small amount of tubes. We looked at the PCR machine in two ways. The first way was reducing the time per cycle. This seemed largely difficult and could diminish the accuracy of the test. The second way was to add more tubes so that the time er cycle would stay the same, but since more tubes were present the amount of tubes tested in a certain time would go up. We decided increasing the number of tubes per test would increase the rate at which tubes are tested. tampering with the cycle time was too risky, and merely adding more tubes was a simple and effective alternative.
Feature 3: Hardware - PCR Machine & Fluorimeter
The PCR and fluorimeter will be separate devices. The fluorimeter will remain unchanged. The PCR was only minimally altered in the consumables area. This was to allow more tubes to be tested. The PCR machine has a specific job, and to try to make it do too many things could sacrifice accuracy. In our case we want the accuracy to be maximized so therefore, only minimal and reasonable changes were made to the PCR machine.
Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach
The calculations show that the PCR is not reliable for predicting the disease, as the result of calculation three and four are significantly less than one. However, PCR's reliability of detecting the disease is very high, shown by the results of calculations one and two being close to 1. With these results, we can conclude that PCR reactions are dependable for detecting the diseased SNP, but are not dependable for predicting the actual occurrence of the disease.