BME100 s2014:T Group2 L6
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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LAB 6 WRITE-UP
In the beginning each part of the PCR machine was imported to Tinkercad. The files represented were 'Body: Back, Bottom, Side, Side with ports, and Top.
Feature 1: Disease SNP-Specific Primers
rs237025 is a SNP(Single nucleotide polymorphism) mainly found in Homo Sapiens. Which means Humans exhibit this variation most often. A nucleotide is basic unit of nucleic acid. A polymorphism is the presence of two or more phenotypes in a population because of the expression of different alleles of a given gene. rs237025 is seen on the 6:149721690 chromosome. Normally, when a disease is classified, they fall under a certain clinical significance. Basically its general effect on day-to-day. That significance could be palpable, real genuine, or a noticeable effect. rs237025 did not fit any of these categories. Diseases closely linked to rs237025 are diabetes and Vogt-Koyanagi-Harada syndrome, a multi-system disease with dermatological effects.
How the primers work: Primers are single strands of DNA that act as the beginning of DNA synthesis. Primers are set to the beginning and end of the desired DNA fragment that will be amplified. Thus this will keep it from amplifying any non-disease alleles.
Feature 2: Consumables Kit
Our PCR system is packaged in a way that makes it easier for the student to get right down to business doing the experiment. It comes complete with PCR mix, primers, PCR tubes, a micropipetter, pipette tips, and an adjustable phone stand, all packaged neatly in a box. The PCR mix and primers are all pre-measured and pre-sorted in the PCR tubes, allowing the experiment to begin as soon as the box is opened. This makes the experimental more efficient overall. Each PCR tube will also be pre-labeled as well as being covered in foil to prevent too much light exposure; also a time saver on the part of the student. This kit is made to increase the efficiency of the experiment, making it more enjoyable for the student.
Feature 3: Hardware - PCR Machine & Fluorimeter
The PCR was run to test DNA samples. While the flourimeter was used find the specifics of flouresence and the intensity of wavelength.
The PCR machine was redesigned in order to save power and electriciy. This was done my removing the LCD screen from the top of the PCR machine. Once the screen was removed the excess wirieng was removed or diverted to processing. Thus, fewer materials are needed to build the machine, and the price will drop significantly. Including a larger well allows more samples to be taken
Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach
According to our results for calculations 3 and 4, we realized that the PCR can not predict diseases very accurately, the reliability is kind of low. Especially for positive result. The numerical value we got in calculation 3 is much smaller than 1. Although the value for calculation 4, which present the negative result is pretty close to 1, but it's still not satisfactory enough. Which means the PCR is not able to verdicts patients' situations well such as false positives cases.