BME100 s2014:T Group1 L6
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LAB 6 WRITE-UP
The design we chose was primarily considered to make the PCR machine more convenient and less bulky. This allowed for the implementation of a bigger display which allowed for more information about the processes going on to be shown on the bigger display (blue screen shown on tinkercad image). Also by using the "slide" implementation,it allows for the samples to be easily accessible when compared to the older design of the PCR machine(the gray tray is the sliding drawer). There are also rubber ends at the corners for durability and a handle on each side of the PCR for convenience.
Feature 1: Disease SNP-Specific Primers
Background on the disease-associated mutation
How the primers work: Primers are complementary DNA sequence for DNA allele, they come in pair, one forward (complementary to the non coding strand) and the reverse primer (complementary to the coding strand). primers bond to the specified allele and the DNA polymerase replicate that specific allele and only that allele because the polymerase can only synthesize molecules with that primer.
Feature 2: Consumables Kit
The problem we had with the consumables previously was that the pipette may have been taking inaccurate measurements because the twisting mechanism was not always precise, we decided that the current pipette was the best fit for our experiments and should not be addressed at this time.
Feature 3: Hardware - PCR Machine & Fluorimeter
Our new design for the PCR machine is a convenient, less bulky design that allows for a bigger, touchscreen display. This way, the screen can display more information for the user to see and understand. We will be implementing a sliding method for the samples that increases the accessibility. The way this works is that the PCR will no longer have a lid but instead have a drawer for the samples that slides in an out of the PCR machine for the user to insert the tubes easily and accurately. In this sliding drawer, we made more holes for the sample tubes. We also made it so that it is much easier to access the inside by reducing the number of screws to remove. Now there are only six screws and once they are removed you can just slide the top right off. Also, for durability, we added rubber ends at the corners of the PCR to absorb any fall damage. Another key component of our machine is the larger base. The larger base allows more surface area for more tubes and faster heat transfer.
Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach
The calculations seem to imply that the PCR is not terribly reliable for predicting positives, as the result of calculation three is much less than one. It is however, slightly more effective in predicting negatives, as the result of calculation four was relatively close to one. The results, in conjunction with one another, would imply that the PCR is not a very reliable way of predicting positive results, or negative results.