OUR AWESOME TEAM
Name: Sean-Christopher Bradbury
Name: Nathaniel Kirkpatrick
LAB 2 WRITE-UP
Initially we tested dosage amounts on the elderly. To format our data we will give it in the format of mean +/- standard error.
LPS Dose of 0 mg had an average of 3.834 +/- 0.4816 pg/ml. The LPS Dose of 5 mg had an average of 8.932 +/- 0.5040 pg/ml. The LPS Dose of 10 mg had an average of 61.622 +/- 9.522 pg/ml. The LPS Dose of 15 mg had an average of 657.941 +/-67.338 pg/ml. Overall, LPS dose of 0 mg had the both the least average inflammotin and the least amount of standard error. The greatest amount of inflammotin average and standard error was in the LPS dose of 15 mg.To back up this information our Anova value was 1.4008E-16 which is well below the .05 cutoff value. The subsequent bonferroni correction results were:
All of these numbers were below .00833333 which was the corrected p-value cutoff.
We chose to do an ANOVA test because there are more than two sample groups. The two-tailed was used because there were multiple subjects rather than one subject tested multiple times. We did a Bonferroni correction for the ANOVA post test to verify that there was a significant change among each of the groups since the overall p-value was below the threshold to be significant.
The second experiment tested the effect of LPS dose (mg) on inflammotin levels (pg/ml) in rats. The data is written as the mean +/- standard error. The LPS dose of 0 mg was 10.515 +/-.9952. The LPS dose of 10 mg was 11.112 +/- 3.31067. Overall, the LPS dose of 0 mg had the smallest amount of average inflammotin and standard error. The t-test value was .8674 which was not below the .05 cutoff to be a significant difference.
We chose to do a t-test because there are two sample groups. The two-tailed was used because there were multiple subjects rather than one subject tested multiple times.
See the graph on the pdf file on Blackboard titled "The Effect of LPS Dose (mg) on Inflammotin Levels (pg/ml) in the Elderly". The y-axis represents the average inflammotin (pg/ml) while the x-axis represents LPS Dose (mg). The four bars represent the different dosage groups. The error bars are standard error, not standard deviation. We provided an insert to better visualize the first three groups which had much more minuscule inflammotin levels in comparison to the 15mg group.
See the graph on the pdf file on Blackboard titled "The Effect of LPS Dose (mg) on Inflammotin Levels (pg/ml) in Rats". The y-axis represents the average inflammotin (pg/ml) while the x-axis represents LPS Dose (mg). The two Ratatouille bars represent the different dosage groups. The error bars are standard error, not standard deviation.
The first experiment was significant even when we did the corrected ANOVA with the Bonferroni test. This leads us to believe that there is a direct link between dosage and the level of inflammotin in the elderly.
The second experiment, which was conducted on rats, did not yield significant results with a p-value of 0.867. We were unable to find a link between dosage and the level of inflammotin among our subjects.
See descriptive statistics section above for a summary and discussion of the results.
While our results proved significant in the trials with the elderly, the rat trials did not. This leads us to believe that the LPS does not have the same effect on rats as it does on the elderly, or at least not in these specific dosages. More data might be necessary to evaluate the effect of LPS on rats.