BME100 s2014:Group31 L1
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LAB 1 WRITE-UP
Independent and Dependent Variables
Independent Variable: Dosage of lipopolysaccharide (mg)
The dosage of lipopolysaccharide is the independent variable because it is the quantity being altered in order to see what the effect of its change will be.
Dependent Variable: Amount of Inflammotin
The amount of Inflammotin protein within the blood stream of test subjects is the dependent variable because it is the quantity that is being measured after varying levels of lipopolysaccharide in order to draw conclusions from.
Each group will have 10 subjects. 10 subjects is a big enough sample size and will help ensure that any extremes will be accounted for.
Among the population of people aged 65 or older (anyone younger will be excluded), a simple random sample will be conducted, which will make the experiment unbiased. Once six groups of ten are randomly selected though the use of drawing names though a hat, each group will be assigned a random dose of lipopolysaccharide. One group will be assigned the control, which is the 10 mg dose of lipopolysaccharide.
Sources of Error and Bias
The people selected for the study might not be representative of the population as a whole. It could be controlled by picking from a larger pool of test subjects, which would make it more likely it represents the population we want to test for as a whole. Another source of error is that some subjects could already have high levels of Inflammotin, so taking a dose of lipopolysaccharide could have a minimal effect. This could be controlled by measuring the subjects' protein level beforehand, and assigning the subjects with high levels to a control group. A final source of error could be that the lowest possible dose of lipopolysaccharide could lie between two whole numbers, but it couldn't be determined since the doses being administered are 1, 3, 5, 7, 9, and 10 milligrams. Controlling this would be difficult as the lab is low on money, so administering more doses could end up costing too much. The lowest dose that is found to increase Inflammotin would then not be the absolutely most precise amount.