BME100 f2016:Group8 W1030AM L5
|BME 100 Fall 2016|| Home |
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Wiki Editing Help
LAB 5 WRITE-UP
PCR Reaction Report
Yes the pre-lab reading helped our group understand what was going on in the lab. The first and second stop were easily figured out, and our group had no problems regarding measuring out the samples and distributing them. The final reactions did not have exactly the same amount of liquid in them due to a small amount of human error but they were very close. There was a very small amount of liquid left inside the tubes that it was inconsequential to the results of this lab. We did not change our labeling scheme throughout the experiment.
-First our group placed the sample onto the slide and turned on the laser. Next, we changed the settings on the Iphone used to match the settings in the lab workbook. Then, we measured out a good distance of approximately 12 cm from the fluorimeter and placed the Iphone on a stand there. The timer on the phone was then set for 5 seconds and the cover was quickly placed on the fluorimeter so the picture could be taken. Lastly, we repeated these steps 3 times for each drop that we placed on the slide.
Data Collection and Analysis
Images of High, Low, and Zero Calf Thymus DNA
Calibrator Mean Values
Images of Our PCR Negative and Positive Controls