BME100 f2016:Group7 W8AM L6
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
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LAB 6 WRITE-UP
Overview of the Original Diagnosis System
There were 14 teams, each containing 4-5 students. Each team diagnosed for two patients, giving a total of 28 patients. In the lab, 3 replicates of the patient's DNA and 3 replicates of positive and negative control were used. ImageJ was used to analyzed the multiple photos. To prevent error and contamination of samples, we used gloves and exchanged plastic, micro pipette tips. PCR controls are indicators of whether the experiment is being conducted correctly. The more drop images taken meant the more accurate and reliable our ImageJ calculations were. Our group took three PCR images for each sample. As a class, the data displayed an equal disbursement of positive and negative conclusions. ImageJ had complications that could have possibly affected our data, but overall, the lab went successful.
What Bayes Statistics Imply about This Diagnostic Approach
Given that the probability of a patient receiving a positive SNP final test and positive PCR reactions, we are able to conclude that calculations 1 showed reliable results. The calculation 2 results imply that the PCR replicate is reliable. Given the results of calculation 3 it is observed that the PCR is unreliable.
Intro to Computer-Aided Design
Feature 1: Consumables
Feature 2: Hardware - PCR Machine & Fluorimeter