BME100 f2016:Group7 W1030AM L5
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LAB 5 WRITE-UP
PCR Reaction Report
Overall the pipetting was fairly straight forward. Luckily, enough of us had worked with a micropipette before and as such the entire process went very smooth. The pre-lab was more or less helpful, maybe not entirely needed, but it did serve it's purpose in ensuring that we were able to get our results transferred as efficiently as possible. Yes we understood the difference (gotta say that it's a pretty nifty feature to have on there. I believe that we were able to successfully transfer over all available liquid, and no we did not have to change our labeling scheme!
The first thing we did was to place a holding apparatus (what could have been a cellphone stand) onto the upper counter top on the lab bench. We then proceeded to place in a cellphone to be used as the recording device for the lab. After ensuring that the cellphone was properly placed, stable, and after measuring the distance from a test drop on the fluorimeter, we then collected some standard images to use as a calibration curve.
Data Collection and Analysis
Images of High, Low, and Zero Calf Thymus DNA
Images of Our PCR Negative and Positive Controls
PCR Results: PCR concentrations solved
PCR Results: Summary