BME100 f2016:Group14 W8AM L5
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LAB 5 WRITE-UP
PCR Reaction Report
The team's experience went very well with pipetting. Everyone in the team got to pipette and familiarize themselves by the end of the period. Yes, the pre-lab reading helped. The first stop is to pick up the solution and the second stop is to expel it. Yes, the final reactions had the same amount of liquid and there was not any liquid left in the tubes. We kept our labeling scheme the same.
To capture images from the flourimeter first the flourimeter was set up by turning it on andplacing a slide in with the smooth side down. We then, set up a phone timer to 3 seconds and turned off the flash. The phone went into a craddle and the flourimeter was lifted with boxes until the slide was in horizontal view of the camera.
Placing Samples onto the Fluorimeter
Data Collection and Analysis
Images of High, Low, and Zero Calf Thymus DNA
Calibration Mean Values
Images of Our PCR Negative and Positive Controls
PCR Results: Summary