BME100 f2016:Group10 W8AM L5

BME 100 Fall 2016	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Alexandra Dalbec Name: student Name: student Name: student Name: student Name: student LAB 5 WRITE-UP PCR Reaction Report Overall the PCR Reaction Report was simple. Before the lab we had an online prelab task to complete which basically showed us how to pipette online. When it came down to the lab we had the knowledge already to complete the pipetting experience because of the prelab. Before my group began pipetting we first labeled our tubes which was a little difficult because the sharpie wasn't showing to well at first. After we got done with pipetting we were a little worried because we noticed there was left over liquid in the bottom of the tubes, we then agreed to go over and pipette the rest of the liquid in the right tubes. Fluorimeter Procedure Imaging set-up First we placed a smartphone the cradle at a right angle form the slide. We then adjusted the height of the fluorimeter by using plastic trays so that the smartphone takes pictures of the droplets at the desired angle, this also helps file out the image. We then adjusted the distance between the smartphone/cradle and the first two rows of slides so that the image can be close as possible without it being blurry. Placing Samples onto the Fluorimeter Place the fluorimeter on the table and turn it on. Then place the slide in the fluorimeter with the smooth side facing down Next we set the camera up by setting the timer to three seconds Then place 80 uL of syber green solution on the slide using a micropipette Then we placed 80 uL sample/calibration solution and injected it on top Next adjust the slide so the light is directly on the drop Place the light box over the fluorimeter along with the smartphone while keeping one flap up Then close the flap before the picture is taken Once the picture was taken remove 160 uL from the slide and properly disposed of it Once again adjust the slide so that the light goes through the circles on there Repeat all the following steps above to collect all five measurements Data Collection and Analysis Images of High, Low, and Zero Calf Thymus DNA Calibrator Mean Values TABLE GOES HEREFile:Mean values Calibration curves Images of Our PCR Negative and Positive Controls PCR Results: PCR concentrations solved TABLE GOES HERE PCR Results: Summary Our positive control PCR result was ____ μg/mL Our negative control PCR result was ____ μg/mL Observed results Patient _____ : Patient _____ : Conclusions Patient _____ : Patient _____ :