PCR reaction mix, 8 tubes, 50 μL each: Mix contains Taq DNA polymerase, MgCl2, and dNTP's.
DNA primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer.
A strip of empty PCR tubes
Disposable pipette tips, each used only once
Cup for discarded tips
Micropipettor
Open PCR machine
PCR Reaction Sample List
Tube Label
PCR Reaction Sample
Patient ID
G2 +
Positive control
none
G2 -
Negative control
none
G2 1-1
Patient 1, replicate 1
13209
G2 1-2
Patient 1, replicate 2
13209
G2 1-3
Patient 1, replicate 3
13209
G2 2-1
Patient 2, replicate 1
26070
G2 2-2
Patient 2, replicate 2
26070
G2 2-3
Patient 2, replicate 3
26070
DNA Sample Set-up Procedure
Step 1
Step 2
Step 3...
OpenPCR program
HEATED LID: 100°C INITIAL STEP: 95°C for 2 minutes NUMBER OF CYCLES: 35
Denature at 95°C for 30 seconds, Anneal at 57°C for 30 seconds, and Extend at 72°C for 30 seconds. FINAL STEP: 72°C for 2 minutes FINAL HOLD: 4°C