BME100 f2014:W Group2 L4

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Owwnotebook icon.png BME 100 Fall 2014 Home
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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OUR TEAM

Name: Victoria Bowman
Role(s)
Name: Andre Dang
Role(s)
Name: Chandler Heaton
Role(s)
Name: Jordan Shinn
Role(s)
Name: Abigail Weiss
Role(s)
Name: Alena Zapata
Role(s)

LAB 4 WRITE-UP

Protocol

Materials

  • Lab coat and disposable gloves
  • PCR reaction mix, 8 tubes, 50 μL each: Mix contains Taq DNA polymerase, MgCl2, and dNTP's.
  • DNA primer mix, 8 tubes, 50 μL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer.
  • A strip of empty PCR tubes
  • Disposable pipette tips, each used only once
  • Cup for discarded tips
  • Micropipettor
  • Open PCR machine


PCR Reaction Sample List

Tube Label PCR Reaction Sample Patient ID
G2 + Positive control none
G2 - Negative control none
G2 1-1 Patient 1, replicate 1 13209
G2 1-2 Patient 1, replicate 2 13209
G2 1-3 Patient 1, replicate 3 13209
G2 2-1 Patient 2, replicate 1 26070
G2 2-2 Patient 2, replicate 2 26070
G2 2-3 Patient 2, replicate 3 26070


DNA Sample Set-up Procedure

  1. Step 1
  2. Step 2
  3. Step 3...


OpenPCR program

HEATED LID: 100°C
INITIAL STEP: 95°C for 2 minutes
NUMBER OF CYCLES: 35
Denature at 95°C for 30 seconds, Anneal at 57°C for 30 seconds, and Extend at 72°C for 30 seconds.
FINAL STEP: 72°C for 2 minutes
FINAL HOLD: 4°C

Research and Development

PCR - The Underlying Technology