BME100 f2014:Group18 L6
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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LAB 6 WRITE-UP
Overview of the Original Diagnosis System
What Bayes Statistics Imply about This Diagnostic Approach
Overall, calculations 1 and 2 imply that the individual PCR replicates were very reliable in concluding that a person has the disease SNP or doesn't. The Bayes value for calculation 1 was very close to 1.00 (100%), and the Bayes value for calculation 2 was also close but not as close as calculation 1. Some possible errors that could have affected the Bayes value in a negative way include definite human error in pipetting the substances correctly in technique and amount of solution. Furthermore, during the fluorimetry, there could have been major errors in blocking out the light correctly thus causing the focusing adjustment of the camera to vary, which would have ultimately affected the images captured. Another source of human error could have been in preparing the solutions correctly and in putting them into the PCR machine correctly. Unlike the results for calculations 1 and 2, the results for calculations 3 and 4 suggest that the PCR is not very reliable in predicting the development of the disease (diagnosis) given a positive or negative test. Calculation 3 had a low probability of predicting the development of the disease correctly while calculation 4 had a higher possibility of predicting the development of the disease correctly with close to a 50% probability of correctly predicting the results. Both Bayes value for calculations 3 and 4 were very small and not close to 1.00 (100%).
Feature 2: Consumables Kit
We will hold the SYBR Green 1 in light blocking packaging which would replace the original tin foil packaging used in the lab. The packaging would also be water proof to prevent cross contamination. The buffers needed to be sterile to assure the cleanliness and accuracy of the lab. The packaging for the micropipettor tips should be cut in half so that they could all be used in one sitting for the lab to allow for the most sterile process.
Feature 3: Hardware - PCR Machine & Fluorimeter
For the fluorimeter, it would be best to include an adjustable camera holder to allow for varying heights to help the camera flush with the side of the PCR machine. We would also change the folding lid of the fluorimeter to allow complete darkness, but still the ability to capture a clear image. The lack of these two features in the existing fluorimeter design, that we experimented with, caused several problems. Without the adjustable camera holder, there were changes in cameras to test who's worked better and provided a clearer image. Eventually, the entire fluorimeter set up had to be redone. Additionally, lowering the flap with the camera on a timer caused a lot of glare and problems because of the camera adjusting to the light. In order to get a clear picture, the lab had to be slightly compromised by allowing the flap to be open partially to at least allow some light to shine into the fluorimeter.