BME100 f2014:Group15 L2

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OUR TEAM

Name: Michael Catchings
Name: Jessica Fong
Name: Ben Heywood
Name: Norihan Elsharawy
Name: Destiny Vidaure
Name: Logan Migliorino

LAB 2 WRITE-UP

Descriptive Statistics


Experiment 1 - Rat Trial


Rat Trial Statistics
LPS Dose (mg) Average Inflammotin Measured (pg/ml) Standard Deviation Standard Error
0mg 10.516 pg/ml 2.225551617 0.995296941
10mg 11.112 pg/ml 7.402885924 3.310671231



Experiment 2 - Human Trial


Human Trial Statistics
LPS Dose (mg) Average Inflammotin Measured (pg/ml) Standard Deviation Standard Error
0 mg 3.834 pg/ml 1.523010177 0.481618106
5 mg 8.932 pg/ml 1.593931547 0.504045412
10 mg 61.622 pg/ml 30.11069386 9.521837451
15 mg 657.941 pg/ml 212.9429762 67.33848166



Results


Experiment 1 - Rat Trial

Rat Trial Results


Experiment 2 - Human Trial

Human Trial Results




Analysis


Experiment 1 - Rat Trial


t-Test: Two-Sample Assuming Equal Variances

Variable 1 Variable 2
Mean 10.516 11.112
Variance 4.95308 54.80272
Observations 5 5
Pooled Variance 29.8779
Hypothesized Mean Difference 0
df 8
t Stat -0.172401575
P(T<=t) one-tail 0.433701749
t Critical one-tail 1.859548038
P(T<=t) two-tail 0.867403497
t Critical two-tail 2.306004135



Experiment 2 - Human Trial


Anova: Single Factor

SUMMARY
Groups Count Sum Average Variance
Column 1 10 38.34 3.834 2.31956
Column 2 10 89.32 8.932 2.540617778
Column 3 10 616.22 61.622 906.6538844
Column 4 10 6579.41 657.941 45344.71112


ANOVA
Source of Variation SS df MS F P-value F crit
Between Groups 3027016.695 3 1009005.565 87.25360195 1.40083E-16 2.866265551
Within Groups 416306.0267 36 11564.0563
Total 3443322.721 39



The p-value for the human study was 1.4e^-16. On the other hand, the rat study produced p-values of .433702 for the first set of data, and a value of .867403 for the second set of data. To put these numbers in context, the human p-value was substantially lower than the ideal p-value of .05, which concludes the test was extremely accurate. For the rat test, the p-value was , in both cases, higher than the ideal value of .05, which shows that this portion of the experiment was inconclusive.



Summary/Discussion


Based on the information gathered in the study, the averages, standard deviation, standard error, counts, and p-values were calculated. For the human trial, a single ANOVA was used to compare four sets of data, and in the rat trail a t-Test was used to compared two sets of data. With the collected data, a graph displaying the average value of inflammotin at each dosage of LPS (0mg, 5mg, 10mg, 15mg), verses the standard deviation was created. The graph of the humans showed that at LPS levels of 0mg, 5mg, and 10mg, the level of inflammotin stayed consistently under 100 pg/ml. Once the dosage was increased to 15mg, the inflammotin was increased to 650pg/ml. In the rat study, the level of inflammotin stayed relatively constant in both groups (0mg, and 10mg). The human study produced better results due to the amount and accuracy of the data. On the contrary, the rat data was inconclusive due to the lack of different dosage trials and inconsistent data. With that being said there is a definite positive correlation between LPS and humans, but not the same for rats.