BISC 219: Mod 2 Media Recipes

Plasmid Isolation and Transformation Solutions

Solution	Composition
Solution 1	50 mM glucose 10 mM EDTA 25 mM Tris, pH 8
Solution 2	0.2 M NaOH 1% SDS
Solution 3	2.7M Potassium Acetate 6.6M Acetic Acid
Luria Broth	1% Tryptone 0.5% Yeast Extract 1% NaCl 2% agar in plates
Ampicillin	Stock = 50 mg/ml in water use 1:1000 in liquid cultures 1:500 in agar
Tetracycline	Stock = 12.5mg/ml in 70% ethanol N.B. tetracycline is light sensitive use 1: 1000 in liquid 1:1000 in agar

RNAi Schedule of Experiments
RNAi General Information
Lab 4: Picking your gene to RNAi
Lab 5: Plasmid DNA isolation and transformation
Lab 6: Induction of RNAi plasmid and C. elegans feeding
Lab 7: Single Worm PCR and Agarose Gel Electrophoresis
Lab 8: PCR Reaction Cleanup and Sequencing