Altman:Protocols/TC Protocols/SF9 conditions

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Department of Physics, Willamette University


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Maintaining SF9 cells

  • Cells are maintained in 125 mL disposable sterile Erlenmeyer flasks in SF-900 II SFM (Gibco) + 100x Pen-Strep-Glutamine (Life Technologies) at 28 C shaking at 150 rpm
  • Once cell culture density has reached >2*10^6 viable cells/mL, the cells are split to a density of 3-5*10^5 viable cells/mL