Altman:Protocols/Preparing Reagents/Making PCR

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Department of Physics, Willamette University

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Making Creatine Phosphate Disodium Salt (PCr)

• Creatine Phosphate Disodium Salt – Calbiochem, Cat #: 2380, 5 gm or 25 gm, store at -20°C, MW is 255.1
• Final stock (100x) should be 100 mM PCr stored in 1x AB

	100 mM PCr
1x AB	3.920 mL
PCr	0.1 g

• Aliquot 50 μL stocks in the cold-room
• Store at -20°C