Difference between revisions of "User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/19"

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(Procedure)
Line 13: Line 13:
 
* This process was repeated three more times to obtain a total of four cell culture-containing falcon tubes.
 
* This process was repeated three more times to obtain a total of four cell culture-containing falcon tubes.
 
* The culture were placed in orbital shaker at 230rpm, at 37°C, for 15 hours.
 
* The culture were placed in orbital shaker at 230rpm, at 37°C, for 15 hours.
* 12.5g of LB media was weighted out and placed in a 250mL Erlenmeyer flask.
+
* 1.25g of LB media was weighted out and placed in a 250mL Erlenmeyer flask.
 
* 50mL of distilled water was weighted out and poured into the Erlenmeyer flask.
 
* 50mL of distilled water was weighted out and poured into the Erlenmeyer flask.
 
* This process was repeated five more times to obtain a total of six LB-containing flasks.
 
* This process was repeated five more times to obtain a total of six LB-containing flasks.

Revision as of 11:29, 5 March 2013

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Purpose

  • To make starter cultures of DH5α-T1 cells and autoclaved LB media for future use.

Procedure

  • One colony of DH5α-T1 cell was inoculated with a sterile wooden stick and dipped into 3mL of sterile LB media inside a 15mL falcon tube.
  • This process was repeated three more times to obtain a total of four cell culture-containing falcon tubes.
  • The culture were placed in orbital shaker at 230rpm, at 37°C, for 15 hours.
  • 1.25g of LB media was weighted out and placed in a 250mL Erlenmeyer flask.
  • 50mL of distilled water was weighted out and poured into the Erlenmeyer flask.
  • This process was repeated five more times to obtain a total of six LB-containing flasks.
  • The flasks were placed in autoclave under liquid cycle.